{"id":17716,"date":"2022-12-01T16:15:00","date_gmt":"2022-12-01T13:15:00","guid":{"rendered":"https:\/\/www.khas.edu.tr\/events\/molekuler-biyoloji-ve-genetik-seminerleri-dr-goker-arpag\/"},"modified":"2025-04-17T16:19:03","modified_gmt":"2025-04-17T13:19:03","slug":"molekuler-biyoloji-ve-genetik-seminerleri-dr-goker-arpag","status":"publish","type":"etkinlik","link":"https:\/\/www.khas.edu.tr\/en\/events\/molekuler-biyoloji-ve-genetik-seminerleri-dr-goker-arpag\/","title":{"rendered":"Molek\u00fcler Biyoloji ve Genetik Seminerleri: Dr. G\u00f6ker Arpa\u011f"},"content":{"rendered":"\n<p>Molek\u00fcler Biyoloji ve Genetik B\u00f6l\u00fcm\u00fcm\u00fcz\u00fcn organize etti\u011fi seminer serisinin yeni konu\u011fu, b\u00f6l\u00fcm\u00fcm\u00fcz\u00fcn akademisyenlerinden\u00a0<strong>Dr. G\u00f6ker Arpa\u011f<\/strong>\u00a0olacak.<\/p>\n\n<p>Dr. Arpa\u011f\u2019\u0131n\u00a0<strong>\u201cCollective effects of XMAP215, EB1, CLASP2, and MCAK lead to robust microtubule treadmilling\u201d<\/strong>\u00a0ba\u015fl\u0131kl\u0131 konu\u015fmas\u0131n\u0131 1 Aral\u0131k Per\u015fembe saat 12:30\u2019dan itibaren B-211\u2019de takip edebilirsiniz.<\/p>\n\n<p>The event is in English.<\/p>\n\n<p><strong>\u00d6zet:\u00a0<\/strong>Microtubule network remodeling is essential for fundamental cellular processes including cell division, differentiation, and motility. Microtubules are active biological polymers whose ends stochastically and independently switch between phases of growth and shrinkage. Microtubule treadmilling, in which the microtubule plus end grows while the minus end shrinks, is observed in cells; however, the underlying mechanisms are not known. Here, we use a combination of computational and\u00a0<em>in vitro<\/em>\u00a0reconstitution approaches to determine the conditions leading to robust microtubule treadmilling. We find that microtubules polymerized from tubulin alone can treadmill, albeit with opposite directionality and order-of-magnitude slower rates than observed in cells. We then employ computational simulations to predict that the combinatory effects of four microtubule-associated proteins (MAPs), namely EB1, XMAP215, CLASP2, and MCAK, can promote fast and sustained plus-end-leading treadmilling. Finally, we experimentally confirm the predictions of our computational model using a multi-MAP,\u00a0<em>in vitro<\/em>\u00a0microtubule dynamics assay to reconstitute robust plus-end-leading treadmilling, consistent with observations in cells. Our results demonstrate how microtubule dynamics can be modulated to achieve a dynamic balance between assembly and disassembly at opposite polymer ends, resulting in treadmilling over long periods of time. Overall, we show how the collective effects of multiple components give rise to complex microtubule behavior that may be used for global network remodeling in cells.<\/p>\n\n<p><strong>Konu\u015fmac\u0131 Hakk\u0131nda:\u00a0<\/strong>G\u00f6ker Arpa\u011f is a visiting academician in the Department of Molecular Biology and Genetics at Kadir Has University. G\u00f6ker obtained his B.S. degree from \u0130stanbul Teknik \u00dcniversitesi Department of Physics in 2011 and his Ph.D. degree in Physics at Worcester Polytechnic Institute in 2016. Although trained as a Physicist, G\u00f6ker\u2019s research has been intersecting with biology. At \u0130T\u00dc, he worked with Prof. Ay\u015fe Erzan to develop statistical and computational methods to study miRNA-mRNA pairing. His strong interest in biophysical research on cytoskeleton and its associated proteins started in graduate school in the group of Assoc. Prof. Erkan T\u00fczel. He developed computational models studying kinesin motility and cargo transport, and force generation by myosins. G\u00f6ker joined Assoc. Prof. Marija Zanic\u2019s laboratory for his postdoctoral training to obtain hands-on experience on biochemical\u00a0<em>in vitro<\/em>\u00a0reconstitution using purified protein components. He used multidisciplinary approaches to study microtubule dynamics and its regulation. G\u00f6ker joined Kadir Has University in September 2022 and is establishing his independent lab. His research interests focus on elucidating fundamental biophysical principles underlying dynamic evolution of microtubule cytoskeleton and microtubule-based transport driven by molecular motors.\u00a0G\u00f6ker will talk about how the collective effects of multiple regulatory proteins give rise to differential regulation of the dynamics at the two microtubule ends, resulting in microtubule treadmilling, a poorly understood cellular behavior.<\/p>\n","protected":false},"featured_media":17714,"template":"","meta":{"slim_seo":{"title":"Molek\u00fcler Biyoloji ve Genetik Seminerleri: Dr. G\u00f6ker Arpa\u011f - Kadir Has University","description":"Molek\u00fcler Biyoloji ve Genetik B\u00f6l\u00fcm\u00fcm\u00fcz\u00fcn organize etti\u011fi seminer serisinin yeni konu\u011fu, b\u00f6l\u00fcm\u00fcm\u00fcz\u00fcn akademisyenlerinden\u00a0 Dr. G\u00f6ker Arpa\u011f \u00a0olacak. Dr. Arpa\u011f\u2019\u0131n\u00a0"}},"birimler":[68],"class_list":["post-17716","etkinlik","type-etkinlik","status-publish","has-post-thumbnail","hentry","birimler-mbg"],"_links":{"self":[{"href":"https:\/\/www.khas.edu.tr\/en\/wp-json\/wp\/v2\/etkinlik\/17716","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.khas.edu.tr\/en\/wp-json\/wp\/v2\/etkinlik"}],"about":[{"href":"https:\/\/www.khas.edu.tr\/en\/wp-json\/wp\/v2\/types\/etkinlik"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.khas.edu.tr\/en\/wp-json\/wp\/v2\/media\/17714"}],"wp:attachment":[{"href":"https:\/\/www.khas.edu.tr\/en\/wp-json\/wp\/v2\/media?parent=17716"}],"wp:term":[{"taxonomy":"birimler","embeddable":true,"href":"https:\/\/www.khas.edu.tr\/en\/wp-json\/wp\/v2\/birimler?post=17716"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}